Regulatory

Part:BBa_K731500:Design

Designed by: Francesco Guzzonato   Group: iGEM12_UNITN-Trento   (2012-08-22)

[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of K731300 + a tac promoter followed by a lac operator. lac operator sequence corresponds to plasmid pET21b one.

tac promoter and lac operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.

Source

BBa_K731300 part + Ptac & lacO from pET21b

References