Regulatory
Part:BBa_K731500:Design
Designed by: Francesco Guzzonato Group: iGEM12_UNITN-Trento (2012-08-22)
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is composed of K731300 + a tac promoter followed by a lac operator. lac operator sequence corresponds to plasmid pET21b one.
tac promoter and lac operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.
Source
BBa_K731300 part + Ptac & lacO from pET21b